Thomas Knotts, Chemical Engineering 1 Background The overall goal of this project was to provide new theories and models that describe DNA hybridization on surfaces on a fundamental level for improved application and design of microarrays. Microarrays work on the principle of DNA hybridization, and can be used to identify the identity or abundance of […]
Search Results for: dna
The Effects of DNA Methylation on Nucleosome Positioning
Marcus Vranes and Dr. Steven Johnson, Molecular Biology Introduction Recent studies have attempted to discover the correlation that exists between DNA methylation and nucleosome positioning, but none have explored the direct effect of DNA methylation on nucleosome formation and positioning. This research is directly testing the effects of DNA methylation on nucleosome positioning, and whether […]
Characterization of the Role of a DNA-Binding Protein on Regulation of DNA Replication in Plant Organelles
Cynthia Perry and Dr. Brent Nielsen, MMBio DNA replication is essential to all life on earth. Not only does replication occur in the nucleus, it also happens in organelles of the cell: the mitochondria and chloroplasts. In higher plants such as Arabidopsis thaliana, the genomes in mitochondria encode proteins which are responsible for DNA replication. […]
Identification of a Consensus DNA Binding Sequence for Nuclear Gdf5
Michael Baldwin and Dr. Laura Bridgewater, Department of Microbiology and Molecular Biology Introduction Nuclear Gdf5 (nGdf5) and nuclear Bmp2 (nBmp2) are newly discovered proteins (1); however, their function has not been determined. They were first found while studying enhancer elements of the Col11a2 gene, and a yeast-one hybrid screen suggested that they could bind to […]
Effect of Epigenetic Modification of DNA on Nucleosome Formation
Steven M. Johnson The following two specific Aims are from my 2011 MEG. We made great progress and completed Aim 1 and modified our Aim 2. Below I will give a status report for each Aim. Aim 1: Perform in vitro nucleosome (invitrosome) reconstitution on naked C. elegans genomic DNA using unmodified recombinant histone octamer […]
Wind Symphony Performance for Western/Northwest CBDNA Regional Conference
Don L Peterson, Music REPORT: The MEG grant funding was designated as $5000 to assist with travel expenses of the Wind Symphony and soloist, Jaren Hinckley and an additional $1500 to purchase digital recorders–for a total of $6500. I am pleased to report on the achievement of objectives sought through this grant. The overall experience […]
The Relationship Of Nd1 Mtdna Gene Polymorphisms To Energy Metabolism And Risk Of Obesity Among Peruvian Native Americans
Karen K. Thomas and Dr. Mark J. Rowe, Food Science and Nutrition INTRODUCTION Mitochondria are involved in the conversion of energy in the cell. Metabolic energy from the food we eat is converted in mitochondria to ATP by the electron transport system (ETS) and oxidative phosphorylation. The enzyme complexes involved in the ETS contain thirteen […]
The Flea Family Tree: Phylogeny Of The Order Siphonaptera Based On 18s rDNA, 28s rDNA, Elongation Factor-1alpha, And Cytochrome Oxidase Ii Gene Regions
Alison Swindle and Dr. Michael F. Whiting, Zoology Fleas are laterally compressed, wingless insects with small helmet shaped heads, and mouthparts specialized for feeding on the blood of their hosts. These morphological specializations make it possible for fleas to be entirely ectoparasitic, but they also make it difficult to find distinguishing characters on which to […]
A STUDY OF RANDOM AMPLIFIED POlYMORPHIC DNA (RAPD) AS MARKERS IN A liNKAGE MAP OF PEROMVSCUS USING TWO INBRED STRAINS
Dan Ririe, Department of Chemistry Introduction Within the mouse genus Peromyscus there exists many pericentric inversions containing linkage groups, genes which do not recombine. It has been hypothesized that genes inherited together provide the selective pressure which has caused speciation within the genus. The purpose of this project was to aid ongoing research in the […]
CHELEX, GUANIDINE, AND PHENOL/CHLOROFORM: A COMPARISON OF EXTRACTIONS FOR ANCIENT DNA FROM DENTIN POWDER
J. Daniel Rasband, Department of Zoology Abstract Several different methods have been employed in the extraction and amplification of ancient DNA (aDNA). The work in this thesis Is an effort to compare three popular extraction techniques, chelex, guanidine/ silica slurry, and phenol/chloroform, as applied to extracting DNA from the dentin of ancient teeth. Modern and […]
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