Julianne H Grose, Microbiology and Molecular Biology Evaluation of how well the academic objectives of the proposal were met Specific Aim 1: Perform yeast two-hybrid screens on wild type CryAB, R120G CryAB, and HspB2 in order to create a protein-protein interaction network and identify potential substrates. Achievements: Large-scale yeast-two hybrid screens were performed on wild […]
Validation of an nBmp2 mutant mouse model
Laura C Bridgewater, Microbiology and Molecular Biology Work in the Bridgewater Lab over the last several years has focused on elucidating the function of a novel protein we discovered, nBmp2. The initial discovery presenting work performed in cultured cells (rather than whole animals) was published back in 2010 and we subsequently constructed a mutant mouse […]
PAS Kinase: A Target for Metabolic Disease
Jordan Mackay and Dr. Julianne Grose, Molecular and Microbiology Many diseases that affect humankind are poorly understood. The medical and scientific communities alike rely heavily on basic scientific research to fuel dissemination of knowledge to said communities for further applied research, including clinical and pharmaceutical efforts. Diabetes is still poorly understood despite its prevalence in […]
PAS Kinase Interactions
Serena Loeb and Dr. Julianne Grose, Microbiology and Molecular Biology Prevalence of metabolic disease, primarily diabetes, is increasing at a steady rate. This trend has created a need for new treatment options. Researchers discovered that the protein PAS (Per-Arnt-Sim) kinase is involved in mammalian metabolic regulation; showing PAS kinase deficient mice exhibited decreased weight gain, […]
Effect of Epigenetic Modification of DNA on Nucleosome Formation
Steven M. Johnson The following two specific Aims are from my 2011 MEG. We made great progress and completed Aim 1 and modified our Aim 2. Below I will give a status report for each Aim. Aim 1: Perform in vitro nucleosome (invitrosome) reconstitution on naked C. elegans genomic DNA using unmodified recombinant histone octamer […]
Molecular Characterization of Pathways involved in PAS Kinase Regulation and Function
Julianne H. Grose Evaluation of academic objectives The academic objectives of this MEG were to provide novel findings in the field of metabolic regulation while training both graduate and undergraduate students. We were able to meet these objectives as evidenced by one published scientific article, one article accepted upon minor revision, and one in preparation. […]
The Role of the KSHV LANA Protein in Development of Persistent Infections in vivo
Bradford Berges Evaluation of how well the academic objectives of the proposal were met Several of my students have moved on to future positions, as follows: Medical school: Marshall Sheide, Stanton Nielsen, German Cuadra Graduate school: Jamie Gardiner, Steve Hallam (still interviewing) Currently employed: Joel Gardner, Wittnee Estes Jamie Gardiner traveled with me to the […]
Targeting IgA Antibody Responses to Mucosal Tissues Through Chemokine Receptor Manipulation
Eric Wilson, Department of Microbiology and Molecular Biology Proper functioning of cells of the immune system is highly dependent on their efficient accumulation to tissues where infection may occur. These cells migrate throughout the body to sites of possible infection via arteries and blood vessels, much like commuters travel to their places of employment via […]
Bax Induced Apoptosis under Control of the Tk1 Promoter
Scott A. Witt and Dr. Kim L. O’Neill, Microbiology The human body has an automatic system for eliminating unwanted cells. When it is time for a cell to be removed, it is given a signal to divide up its parts into discrete packages and send them to other cells to be recycled. The cell eventually […]
Molecular Mechanisms of Lupus Risk Conferred by Allele in the Gene for Interferon Response Factor 5
Dr. Brian Poole, Department of Microbiology & Molecular Biology Evaluation of how well the academic objectives of the proposal were met The academic objectives of this proposal were met. Using the funding provided by the MEG, we generated data that was used to publish four peer-reviewed journal articles and one peer-reviewed book chapter. These are: […]